We have set up a publicly accessible web-server of TPpred-LE. To facilitate the users, a step-by-step guide about how to use the web-server was described below:

  Step 1. Input the query peptide sequence in FASTA format into the input box or click the Choose File button to upload a file that contains sequences in FASTA format. The examples of peptide sequences in FASTA can be obtained by clicking Example button right above the input box. As the server needs to generate PSSM for every query sequence by running PSI-BLAST, which is a time-consuming process. You can input your valid Email address if you want to see the results in your email.

  Step 2. Click on the Submit button to submit the task, then you will get the predicted results on your screen or receive an email that contains the predicted results (if you filled in the email).

  Step 3. If you need to obtain the datasets used in this study, you can click on the Materials navigation bar.